Figure 4.
CD47 blocking antibody increases the number of microglia in ipsilateral basal ganglia. (a) Representative native EGFP (enhanced green fluorescent protein; green) fluorescence images of different brain regions from a coronal section of Tmem119-EGFP in ICH+anti-CD47 group and ICH + IgG group 3 days and 7 days post hemorrhage. Regions of interest were hematoma core, peri-hematoma, ipsilateral basal ganglia (BG) areas, and contralateral BG. (b) Quantification data on the number of GFP(+) cells in different brain regions 3 days after hemorrhage and (c) Quantification data on the number of GFP(+) cells in different brain regions 7 days after hemorrhage. *P < 0.05 vs the ICH + IgG group. **P < 0.001 vs the ICH + IgG group. Scale bars are 50 µm. Values are means ± SD or median (quartile 1, quartile 3); n = 6 per group.
