Table 1. Summary culture methods. Methods were implemented sequentially 15 months in 230–460 L culture tanks.

For each, tanks were continuously setup and torn down (cycled) to maintain fouling environments of similar age and constituency between tanks.

Husbandry method	Time frame	# Months tested	# Tank replicates /method	# Genotypes	Maintenance hours /tank/day
Mini Reef	Feb 13–May 27, 2020	4	1	3	0.25–0.50
Clean Start	Apr 1–Oct 5, 2020	7	6	17	1.0–4.0
Green Film Summer	Sep 11-Dec 21, 2020; Apr 1–Sep 13, 2021	8.5	9	29	0.25–1.0
Green Film Winter	Dec 4, 2020–Apr 26, 2021	5	6	21	0.25–1.0