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. 2023 Aug 7;11:e15723. doi: 10.7717/peerj.15723

Table 2. Advantages and disadvantages of each culture method for restoration.

Culture Method Advantages Disadvantages
Mini Reef • Familiar strategy
• Approximates fouling of a reef
• Low maintenance time once established (0.25–0.50 h/tank/day)
• Minimizes algal overgrowth of microfragments
• No tank restarts needed
• Waiting required—lengthy and variable establishment times (1 + yr)
• Slowest grow out time achieved here
• Cyanobacteria potentially problematic
• Harbors an irreplicable mixture of foulers, potentially resulting in a tank effect
Clean Start • Easy to start, no waiting
• Reduced subset of foulers to manage due to early settlement dynamics
• Fouling actively managed
• Establishes CCA quickly
• Fast grow-out time
• High maintenance time (1–4 h/tank/day)
• Threat of nuisance algae bloom is high throughout the tank’s life
• Restart required in 4–6 months to avoid mini reef conditions
Green Film • Preselects for benign green algae
• Low maintenance time (0.25–1 hour/tank/day)
• Simple, replicable fouler management
• Fastest grow-out time
• Nuisance algae blooms are infrequent
• Two-week waiting period for film to develop
• Restart required in 4–6 months to avoid mini reef conditions