Figure 2.

HOTAIR affects the invasion and metastasis of liver cancer cells. (A) Tested the transfection efficiency of Sh-HOTAIR plasmid. (B-C) The transwell experiment detected the invasion ability of cells. (D-E) Scratch test detected cell migration ability. (F) Detected the relationship between HOTAIR and EMT related proteins by Western blot. (G) qPCR experiment to detect the RNA expression levels of E-cadherin, N-cadherin, Vimentin, MMP2, MMP9 genes. (H) Detected the protein expression level of N-cadherin by immunofluorescence method. Added arrows to indicate cells of interest. The data shown were representative of three independent experiments. Bars, SD (n=3), *p<0.05, **p<0.01, and ***p<0.001 vs NC group.