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. 2023 Jul 24;14(12):2329–2343. doi: 10.7150/jca.85335

Figure 7.

Figure 7

HOTAIR regulates the expression of NUAK1 through miR-145-5p. (A-B) Transfected Sh-HOTAIR and LZRS-HOTAIR plasmids in SNU-387 and HepG2, and detected the expression level of miR-145-5p by qPCR. (C) Transfected si-EZH2 in SNU-387 and HepG2, and detected the expression level of miR-145-5p by qPCR. (D)Transfected LZRS-HOTAIR plasmid in SNU-387 and HepG2, and transfected LZRS-HOTAIR plasmid while adding si-EZH2, and detected the expression level of miR-145-5p by qPCR. (E-H) ChIP assays in SNU-387 and HepG2 transfected with sh-HOTAIR and si-EZH2 cells were performed on the miR-145-5p promoter regions using anti-H3K27me3 and EZH2 antibodies. Enrichment was determined relative to the input controls. (I)Transfected Sh-HOTAIR plasmid in SNU-387 and HepG2, and transfected Sh-HOTAIR plasmid while adding miR-145 inhibitor, Western blot experiment to detect the protein expression of NUAK1. The data shown were representative of three independent experiments. Bars, SD (n=3), *p<0.05, **p<0.01, and ***p<0.001 vs NC group.