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. 2023 Jul 16;14(12):2224–2235. doi: 10.7150/jca.85650

Figure 2.

Figure 2

KMU-191 induces apoptosis and regulates apoptosis-related proteins in cancer cells. (A,C) Caki cells were treated with the indicated concentrations of KMU-191 for 24 h. (B,D) Caki cell were treated with 10 μM KMU-191 for the indicated time points. (E,F,G) A549, p53 wild-type HCT116, and p53 deficient HCT116 cells were treated with the indicated concentrations of KMU-191 for 24 h, respectively. Cell cycle populations was measured by flow cytometry (A,B,G). Sub-G1 populations and protein expression were measured by flow cytometry (C-F) and Western blotting analysis (C,D), respectively. Cleavage of PARP is indicated with an arrowhead. The expression level of β-actin was used as a control of protein loading. Values in the graph (A-G) indicate the mean ± SD of three independent experiments. * P < 0.05 compared to the respective control. HCT116 p53 (+/+), p53 wild-type HCT116 cells; HCT116 p53 (-/-), p53 deficient HCT116 cells.