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. 2023 Jul 16;14(12):2289–2300. doi: 10.7150/jca.85906

Figure 2.

Figure 2

FOXP1 responsible for GINS1 expression in DLBCL. (A) Nucleotide sequence of the promoter region of GINS1 gene. Two predicted FOXP1 binding sites (site A and site B) were shown. +1 indicates the position of the transcription initiation site of the GINS1 gene. (B) Left: the schematic diagram of the luciferase reporter constructs containing the indicated genomic fragments of GINS1 gene was shown. Right: the results of the luciferase reporter assay in SU-DHL-2 cells. C. Co-transfected luciferase reporter constructs with pcDNA3.1-FOXP1. D. Co-transfected luciferase reporter constructs with shFOXP1 expressing plasmids. E. The recombinant FOXP1 were expressed and purified, then analyzed by SDS-PAGE. F. EMSA analysis with the SU-DHL-2 nuclear extract or recombinant FOXP1 protein. (G) ChIP assay. Agarose gel electrophoresis of ChIP products (***P<0.001).