FIG. 6.

FIV entry. (A) Infection of primary feline PBMC and CrFK cells by virions pseudotyped with the envelope glycoprotein from primary and adapted FIV or the G protein of VSV. The transduction of PBMC was performed with approximately 20, 400, and 15 ng of p24 for primary FIV envelope glycoprotein, adapted FIV glycoprotein envelope, and G protein, respectively. The transduction of CrFK cells was performed with approximately 200, 800, and 15 ng of p24 for primary FIV envelope glycoprotein, adapted FIV envelope glycoprotein, and G protein, respectively. Results for feline PBMC (white bars) and CrFK cells (crosshatched bars) are means ± standard errors of the mean (error bars) for triplicate wells. RLU, relative light units. (B) Effects of CXCR4 ligands on entry. The transduction of primary feline PBMC by virions pseudotyped with the envelope glycoprotein from a primary FIV isolate (panel i) or the G protein of VSV (panel ii) was examined in the presence of SDF-1α, the bicyclam AMD3100, or peptide C9W. Luciferase activity (% infection) is expressed as a percentage of luciferase activity without an inhibitor. Results are means (M) ± standard errors of the mean (error bars) for triplicate wells.