FIG. 6.

Expression of MCK-2 in fibroblasts infected with MCMV. NIH 3T12 fibroblast cells were mock infected or infected with MCMV at multiplicity of infection of 5, and cells were harvested at the indicated times after infection. An aliquot of each sample was subjected to SDS–12.5% PAGE and Western analysis using anti-MCK-2 monoclonal antibody 5A5. Lanes 1 through 10 contain NIH 3T12 cell samples. Lanes 11 and 12 show for comparison proteins present in COS 1 cells transfected with vector pmt21 or the MCK-2 expression construct pmt21MCK-2-cStop, respectively. Sizes are indicated in kilodaltons.