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. 2023 Aug 8;10(8):ENEURO.0204-23.2023. doi: 10.1523/ENEURO.0204-23.2023

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Copyright © 2023 Hostetler et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 4. — Retrograde FB labeling. A, Representative 30-μ;m-thick section from a retrogradely labeled Pdyn mouse. Image is a projection of a z-stack taken with a 20×, 0.75 NA objective at 2.5 μ;m z-steps; color channels were adjusted individually in each panel. Far left panel shows only the FB color channel, and the adjacent panel also shows GFP and tdTomato channels. The 4 boxed ROIs are shown enlarged to the right, separated into the FB channel (left panels) and GFP-tdTomato channels (right panels). Filled arrows point to all FB⁺ SOM cells in each ROI, hollow arrows point to all FB^– SOM cells. B, Top panels, Number of FB⁺ and FB^– GFP⁺ cells as a percentage of all GFP⁺ cells counted in each brain, averaged by genotype. Bottom panels, Same numbers as a percentage of all GFP⁺ cells in each layer. Error bars are the SEM. Plots at the far right show the fraction of FB⁺ cells of all SOM cells in all genotypes. The number of animals is as in Figure 1. Numerical data are provided in Table 4.