Table 5.
Marker protein immunoreactivity in the 4 intersectional subsets
| L2/3 | L4 | L5 | L6 | All layers | ||
|---|---|---|---|---|---|---|
| Calb2 (N = 3) | ||||||
| CR+ | % of all GFP+ | 38.2 (3.7) | 4.8 (4.8) | 24.3 (3.7) | 5.7 (1.8) | 73.1 (4.6) |
| % in layer | 69.3 | 88.9 | 83.2 | 55.9 | 73.1 | |
| NPY+ | % of all GFP+ | 53.1 (2.0) | 4.4 (1.5) | 20.6 (6.5) | 4.4 (1.5) | 82.5 (8.6) |
| % in layer | 83.4 | 76.5 | 83.5 | 75.0 | 82.5 | |
| CB+ | % of all GFP+ | 31.3 (4.0) | 7.1 (2.6) | 20.7 (5.3) | 2.4 (1.8) | 61.5 (0.5) |
| % in layer | 53.0 | 93.9 | 75.8 | 40.4 | 61.5 | |
| Chrna2 (N = 3) | ||||||
| CR+ | % of all GFP+ | 0.4 (0.4) | 1.0 (0.6) | 1.4 (0.7) | ||
| % in layer | 0.5 | 6.0 | 1.4 | |||
| NPY+ | % of all GFP+ | 0.0 (0.0) | 4.3 (3.7) | 5.1 (2.0) | 9.4 (4.7) | |
| % in layer | 0.0 | 5.6 | 23.3 | 9.4 | ||
| CB+ | % of all GFP+ | 58.6 (4.7) | 8.5 (1.2) | 67.1 (3.7) | ||
| % in layer | 67.4 | 64.9 | 67.1 | |||
| Calb1 (N = 3) | ||||||
| CR+ | % of all GFP+ | 11.7 (1.7) | 1.9 (0.3) | 4.8 (0.6) | 0.8 (0.3) | 19.2 (2.3) |
| % in layer | 61.0 | 26.4 | 8.9 | 4.0 | 19.2 | |
| NPY+ | % of all GFP+ | 15.3 (1.6) | 2.9 (0.7) | 10.4 (3.8) | 9.4 (3.3) | 38.0 (9.2) |
| % in layer | 76.2 | 42.5 | 19.7 | 46.3 | 38.0 | |
| CB+ | % of all GFP+ | 11.9 (2.8) | 2.7 (0.6) | 28.7 (4.2) | 15.2 (1.8) | 58.6 (5.6) |
| % in layer | 62.9 | 37.4 | 55.9 | 67.9 | 58.6 | |
| Pdyn (N = 3) | ||||||
| CR+ | % of all GFP+ | 6.6 (0.9) | 1.4 (0.3) | 4.4 (0.5) | 0.5 (0.3) | 13.0 (1.5) |
| % in layer | 42.3 | 7.0 | 8.0 | 6.1 | 13.0 | |
| NPY+ | % of all GFP+ | 6.3 (1.3) | 5.3 (1.3) | 6.7 (1.4) | 5.2 (0.5) | 23.5 (3.6) |
| % in layer | 54.6 | 28.1 | 10.9 | 66.3 | 23.5 | |
| CB+ | % of all GFP+ | 10.6 (2.3) | 2.0 (0.4) | 16.4 (0.7) | 3.1 (0.4) | 32.0 (2.0) |
| % in layer | 60.6 | 8.7 | 31.1 | 41.6 | 32.0 | |
| All SOM | ||||||
| CR+ (N = 12) | % of all SOM | 7.3 (0.6) | 1.0 (0.2) | 3.8 (0.3) | 1.5 (0.1) | 13.6 (0.9) |
| % in layer | 51.5 | 9.0 | 7.4 | 6.4 | 13.6 | |
| NPY+ (N = 12) | % of all SOM | 9.7 (1.0) | 3.4 (0.6) | 9.4 (1.4) | 10.7 (0.9) | 33.3 (3.0) |
| % in layer | 71.4 | 30.6 | 18.7 | 43.3 | 33.3 | |
| CB+ (N = 12) | % of all SOM | 7.9 (0.6) | 1.7 (0.2) | 20.0 (0.9) | 10.3 (0.6) | 39.9 (1.4) |
| % in layer | 51.6 | 13.8 | 40.5 | 44.5 | 39.9 |
For each antibody, the top row indicates the count of immunostained cells in each layer, expressed as a percentage of all GFP+ cells in the same sections. The SEM is indicated in parenthesis. The bottom row expresses the same counts as a percentage of all GFP+ cells in the same layer. Empty cells indicate that no GFP+ neurons were found. N = 3 mice for each antibody in each genotype; most brains were used for 2–3 antibodies each. The bottom block in the table indicates counts of immunostained cells expressed as a percentage of all SOM cells (GFP+ and tdTomato+) counted in each brain, averaged over all genotypes, N = 12 mice/antibody. These data are plotted in Figure 6.