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Titration of the amount of E2-expressing plasmid cotransfected with the CAT plasmid driven by the grp94 promoter into HuH-7 cells. The total amount of transfected plasmids was held constant by using pUC19. The results are normalized to CAT expression in the presence of pUC19 only and are shown as the means and standard deviations from three transfections.
