FIG. 3.

Sucrose-purified recombinant virions. Cells infected with recombinant viruses were metabolically labeled with ³⁵S translabel. Supernatants were collected, and viruses were purified by centrifugation through 20% sucrose. The volumes loaded were normalized for N-P protein content. Lane 1, 1% of total VSV-HA virus from a 3.5-cm-diameter dish of BHK cells; lane 2, 18% of total CT1-HA virus from a 3.5-cm-diameter dish of BHK cells, lane 3, 14% of total VSVΔG-HA virus from a 3.5-cm-diameter dish of BHK-G cells; lane 4, 13% of total VSVΔG virus from a 3.5-cm-diameter dish of BHK-G cells.