TABLE 1.
HIV-1 infectivity-to-virion particle ratiosa
| Protease variant | TCID50/ml | p24 antigen (ng/ml) | TCID50/p24 antigen ratio |
|---|---|---|---|
| NL4-3 (WT) | 45,275 (12,999) | 455 (54) | 100 |
| D30N | 8,947 (1,253) | 305 (18) | 29 |
| D30N/L63P | 30,667 (15,858) | 266 (4) | 115 |
| L90M | 32,700 (8,250) | 453 (26) | 72 |
| L63P/L90M | 32,467 (16,267) | 408 (10) | 79 |
| L10R/M46I/L63P/V82T/I84V | 64,967 (33) | 498 (13) | 130 |
a
Infectivity was measured by determining TCID50, and virion particles were estimated by HIV-1 p24 antigen measurement from a single clarified supernatant (virus stock) of each HIV variant. All assays were performed in triplicate, and the standard error is in parentheses.