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. 2023 Jul 27;14:1229950. doi: 10.3389/fmicb.2023.1229950

Table 1.

Primers and PCR cycling conditions used in the current study.

Gene	Sequence (5-3)	Amplicon size	Cycling conditions	References
cox1	HCO2198: TAAACTTCAGGGTGACCAAAAAATCA	710 bp	$\frac{9 8^{•} C}{30 s e c} \to 40 X [\frac{9 8^{•} C}{10 s e c} \to \frac{6 3^{•} C}{20 s e c} \to \frac{7 2^{•} C}{25 s e c}] \to \frac{7 2^{•} C}{5 m i n} \to 1 0^{•} C$	Folmer et al., 1994
cox1	LCO1490: GGTCAACAAATCATAAAGATATTGG	710 bp		Folmer et al., 1994
GroEL	CoxGrF1: ^* TTTGAAAAYATGGGCGCKCAAATGGT	655 bp	$\frac{9 5^{•} C}{3 m i n} \to 30 X [\frac{9 5^{•} C}{30 s e c} \to \frac{5 6^{•} C}{30 s e c} \to \frac{7 2^{•} C}{1.5 m i n}] \to \frac{7 2^{•} C}{7 m i n} \to 1 0^{•} C$	Duron et al., 2014
	CoxGrR2: ^* CGRTCRCCAAARCCAGGTGC	655 bp
	CoxGrF2: ^** GAAGTGGCTTCGCRTACWTCAGACG	619 bp
	CoxGrFR1: ^** CCAAARCCAGGTGCTTTYAC	619 bp

^*

First run of nested PCR.

^**

Second run of nested PCR.