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FFPE-DNA fragment size (left) and DIN (right) correlate with NGS coverage uniformity (Fold 80 base penalty). DNA fragment size and DIN were determined on a gel electrophoresis system. Fold 80 base penalty was determined bioinformatically after sequence alignment. This correlation is based on 53 identically prepared whole exome sequencing libraries. Perfect coverage uniformity is defined by Fold 80 base penalty value of 1.
