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. 2023 Jun 28;51(14):7675–7690. doi: 10.1093/nar/gkad503

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Description and functional characterization of RolR resorcinol biosensors in Escherichia coli. (A) Structure of the one-plasmid RolR biosensor genetic circuit. RolR expression is driven by the IPTG-inducible tac promoter (regulatory module), and GFP expression is driven by a second engineered resorcinol-responsive promoter (reporter module), which contains one copy of the rolO operator sequence inserted immediately downstream of the –10 promoter element. (B) Left panel: gradually increasing IPTG concentrations reduces whole-cell OD-normalized fluorescence levels. Right panel: Gradually increasing resorcinol concentrations in fixed IPTG concentrations results in an increase in OD-normalized fluorescence levels, and resorcinol EC₅₀ values in different IPTG concentrations were measured. (C) Ligand-specificity of RolR towards all molecules in this study in the presence of 1 mM IPTG. Data points are mean ± SD, n = 3. A.U., arbitrary units.