Figure 4.

First generation variant hits towards catechol. (A) Table depicting a subset of identified variants responsive to catechol, their substitutions coloured according to group, and their response towards resorcinol (RCO) and catechol (CAQ). Variants highlighted in yellow were used as a template for further mutagenesis. (B) Dose response curves of the top-performing variants compared to WT RolR with catechol (left panel) and resorcinol (right panel). (C) Dynamic range of WT RolR and identified variants. (D) Position of residues mutated in the RolR binding pocket around resorcinol using PDB:3AQT. Data points are mean ± SD, n = 3. A.U., arbitrary units.