Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jun 28;51(14):7288–7313. doi: 10.1093/nar/gkad538

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 5. — Transcriptomic analysis of the effects of Mediator kinases on signal-regulated gene expression. (A–D) RNA-Seq analysis of 293 cells treated with the indicated signals in the presence or in the absence of 1 μM Senexin B (SnxB), added 1 h before signal stimulation and maintained till the end of experiment. (A) Cells were serum starved for 48 h and then treated with serum (FBS added to 10% final concentration) for 30 min. (B–D) Cells were treated with TNF (10 ng/ml) for 2 h (B) or PMA (30 nM) for 2 h (C) or 24 h (D). The dot plots show the effects of Senexin B treatment on the signal-affected DEGs (FC > 1.5; FDR < 0.05). Red dots: Senexin B-affected DEGs. Blue dots: Senexin B-unaffected DEGs. The tables on the right show the number and percentage of signal-regulated DEGs affected by Senexin B treatment. (E–H) Comparison of effects of Senexin B on the expression of genes regulated by Senexin B either under basal conditions or upon signal stimulation. Red circles: signal-regulated genes. Blue circles: genes that are not regulated by signals. (I) Effects of different signals on the expression of 46 DEGs regulated by Senexin B at 3 h time point under basal conditions. (J–L) qPCR analysis of mRNA expression of the indicated genes in dKO derivatives with or without signal or Senexin B: serum stimulation (J), TNF (K), PMA (24 h) (L). Data are presented as mean ± SEM (n = 3). Asterisks: P < 0.01 (two-way ANOVA, Tukey's multiple comparisons test) for the differences between Senexin B-treated and untreated conditions.