Skip to main content
. 2023 Aug 10;137(15):1195–1208. doi: 10.1042/CS20230164

Figure 5. Mpv17 knockdown in cultured β-cell, MIN6, protected against PA-induced injury and apoptosis.

Figure 5

(A) Cell real-time assay showed attenuated decrease in viability of the cells treated with si-Mpv17 followed by PA. The result is presented with mean ± SD of the replicate wells. *P<0.05, statistically significant; **P<0.01, statistically very significant. The blue stars refer to the comparison of Scr+Veh vs. Scr+PA, yellow stars to si-Mpv17+Veh vs. si-Mpv17+PA, turquoise stars to Scr+PA vs. si-Mpv17+PA, and red stars to Scr+Veh vs. si-Mpv17+Veh. (B) Condensation nuclei counting showed attenuated apoptosis in the cells with Mpv17 knockdown in panel (A) compared with the control. The result is the mean ± SD of the counts of condensation nuclei from 20 random fields. (C) Immunoblotting shows that cleaved caspase 3 was reduced in the cells with Mpv17 knockdown in the treatment of PA. The results are presented with mean ± SD from three independent experiments. The comparisons between groups were performed using two-way ANOVA followed by Bonferroni’s post-hoc test (B). *P<0.05, statistically significant; **P<0.01, statistically very significant.