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. 2023 Jul 3;25(8):1157–1172. doi: 10.1038/s41556-023-01180-2

Extended Data Fig. 2. yArf1-11 is a hyperactive mutant present on the ER and LDs.

Extended Data Fig. 2

(A, B) Co-localization of yArf1-GFP (A) and yArf1-11-GFP (B) with the cis-Golgi marker Mnn9-mCherry grown at 23 °C or shifted to 37 °C. Arrows indicate sites of co-localization between yArf1/yArf1-11 and Mnn9. Scale bar: 5 µm. (C, D) Co-localization of yArf1-GFP (C) and yArf1-11-GFP (D) with the trans-Golgi marker Sec7-mCherry and Tvp23-mCherry grown at 23 °C or shifted to 37 °C. Arrows indicate sites of co-localization between yArf1/yArf1-11 and the corresponding markers. (E, F) Transmission electron microscopy of the yArf1-11-GFP strain grown either at 23 °C (E) or shifted to 37 °C for 30 min (F). yArf1-11-GFP localizations were highlighted by immunogold labelling, and dotted squares shows enlargements of yArf-11 specific localizations (arrows). Scale bar: 500 nm, scale bar magnification: 200 nm. (G, H) Co-localization of yArf1-GFP and yArf1-11-GFP with the COPI markers Sec21-mCherry (G) and Sec27-mCherry (H) grown at 23 °C or shifted to 37 °C. Arrows indicate sites of co-localization between the yArf1 or yArf1-11 and COPI components. Scale bar: 5 µm.