Extended Data Fig. 9. yArf1 is present at organellar contact sites.

(A–C) High-resolution microscopy of yArf1-CFP strain expressing Erg6-YFP as LD marker, Pex3-mCherry as peroxisomal marker and MitoTracker Deep Red FM to stain for mitochondria. Cells were grown at 23 °C or shifted to 37 °C for 30 min. Localizations of LD and yArf1 (A), peroxisomes and yArf1 (B), or LD-peroxisomes and yArf1 (C) at the vicinity of mitochondria were established by following individual fluorescent intensities of each markers on a 1.5-3 µm distance. Representative images are shown and fluorescent intensities measured along the dotted lines. Single planes of 0.2 µm thickness are shown. Scale bar: 2 µm.