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. 2023 Jul 3;25(8):1157–1172. doi: 10.1038/s41556-023-01180-2

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, TEM of yARF1 and yarf1-11 strains grown at 37 °C for 30 min. Scale bars, 2,000 nm. b, LipidTox staining of LDs in yARF1 and yarf1-11 strains grown at 23 °C or shifted to 37 °C. Scale bar, 5 µm. Mean and standard deviation are shown; yARF1 23 °C = 408 cells, yARF1 37 °C = 408 cells, yarf1-11 23 °C = 421 cells, yarf1-11 37 °C = 422 cells from n = 3 biological replicates; two-way ANOVA using Sidak’s multiple comparison, ***P = 0.0009, **P = 0.0032. c, Nile Red staining of LDs in parental HeLa cells (control), ARF1 KO HeLa cells, and ARF1 KO HeLa cells expressing mArf1 or mArf1-11. For each cell line, the numbers of LD were quantified. Images were acquired 24 h after transfection. Mean and standard deviation are shown; HeLa control = 182 cells, ARF1 KO = 181 cells, ARF1 KO +mArf1 = 163 cells, ARF1 KO +mArf1-11 = 151 cells from n = 3 biological replicates; unpaired two-tailed t-test, **P = 0.0096. Scale bar, 5 µm. d, Measurements of TAG in the yARF1 and yarf1-11 strains grown at 23 °C or shifted to 37 °C. Mean and standard deviation are shown from n = 3 biological replicates; unpaired two-tailed t-test, *P = 0.0349; **P = 0.002. e, LDs (Erg6) and mitochondria (Tom70) morphologies imaged in the yARF1 and yarf1-11 parental strains and in strains deprived of SCS3 and YFT2 (∆scs3 ∆yft2) grown at 23 °C or shifted to 37 °C. f, LipidTox staining of LDs in WT and ∆arf1 strains grown at 23 °C or shifted to 37 °C. Mean and standard deviation are shown; WT 23 °C = 669 cells, WT 37 °C = 662, ∆arf1 23 °C = 673 cells, ∆arf1 37 °C = 659 cells from n = 3 biological replicates Two-way ANOVA using Sidak’s multiple comparison, WT versus ∆arf1 23 °C **P = 0.0032, WT versus ∆arf1 37 °C **P = 0.001. g, Measurements of TAG in the WT and ∆yarf1 strains grown at 23 °C or shifted to 37 °C. Mean and standard deviation are shown from n = 3 biological replicates; unpaired two-tailed t-test, ***P = 0.0002; ****P = 0.00000396. Source numerical data are available in source data. See also Extended Data Fig. 4.

Source data