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. 2023 Jul 13;25(8):1101–1110. doi: 10.1038/s41556-023-01178-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 6 — Ratiometric fluorescence measurement of Keima–spartin in SUM159 wild-type cell in various conditions annotated below the graph. Cells were treated with 0.5 mM OA for 24 h and chased for 4 h after OA withdrawal together with metabolic perturbations (100 nM Baf A1 treatment, amino acid depletion, HBSS, or 250 nM Torin 1 addition). Median ± the 25^th to 75^th percentiles, the whiskers extended to the minima and the maxima, n = 11 cells (Baf A1), 12 cells (OA withdrawal), 10 cells (AA depletion), 10 cells (HHBS), and 11 cells (Torin1) from two independent experiments. Source numerical data are available in source data.

Source data