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. 2023 Jul 13;25(8):1101–1110. doi: 10.1038/s41556-023-01178-w

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Strategy for the knockout of SPG20/spartin. Individual rectangular bar represents exon (gray color, untranslated region; black color, translated region). An sgRNA target sequence is shown with PAM sequence. b, Immunoblot analyses of SUM159 cells, wild-type and cells lacking spartin. c, Endogenous spartin interacts with endogenous LC3. Endogenous spartin was immunoprecipitated with anti-spartin antibody in SUM159 wild-type cells and analyzed by immunoblot with anti-spartin, anti-LC3, and anti-PLIN3 antibodies. * denotes IgG heavy change. d, Immunoblot analyses of SUM159 cells wild-type and cells lacking ATG5, ATG7, or FIP200. Unprocessed blots are available in source data.

Source data