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. 2023 Jul 7;31(8):101701. doi: 10.1016/j.jsps.2023.101701

Table 1.

Acute toxicity study of the aqueous extract of Ferula communis administered orally and intraperitoneally in mice.

Dose (g/kg BW) Sex Administration route
Oral
Intraperitoneal
D/T Latency Toxic symptoms D/T Latency Toxic symptoms
Control Male 0/5 None 0/5 None
Female 0/5 None 0/5 None
0.1 Male 0/5 None 0/5 None
Female 0/5 None 0/5 None
0.5 Male 0/5 None 0/5 None
Female 0/5 None 0/5 None
1.0 Male 0/5 None 0/5 None
Female 0/5 None 0/5 None
1.5 Male 0/5 None 1/5 > 48 h, < 72 h Ataxia, hypoactivity,
Female 0/5 None 1/5 > 48 h, < 72 h Ataxia, hypoactivity,
2.0 Male 0/5 None 2/5 > 48 h, < 72 h Anorexia, Ataxia, hypoactivity, piloerection.
Female 1/5 > 72 h, < 96 h Anorexia, Ataxia, hypoactivity,piloerection 3/5 > 24 h, < 48 h Anorexia, Ataxia, hypoactivity, piloerection.
2.5 Male 0/5 None 2/5 > 24 h, < 48 h Anorexia, Ataxia, hypoactivity, piloerection, asthenia, convulsions.
Female 1/5 > 72 h, < 96 h Anorexia, Ataxia, hypoactivity, piloerection. 3/5 < 12 h Anorexia, Ataxia, hypoactivity, piloerection, asthenia, convulsions.
3 Male 0/5 None 3/5 < 12 h Anorexia, Ataxia, hypoactivity, piloerection, Lethargy, asthenia, asthenia, convulsions,
Female 2/5 > 48 h, < 72 h Anorexia, Ataxia, hypoactivity, syncope. 5/5 < 12 h Anorexia, Ataxia, hypoactivity, piloerection, Lethargy, asthenia, convulsions, syncope.
Dose (g/kg BW) Sex Administration route
Oral
Intraperitoneal
D/T Latency Symptoms D/T Latency Symptoms
3.5 Male 0/5 None 4/5 < 12h Anorexia, Ataxia, hypoactivity, piloerection, Lethargy, asthenia, convulsions, syncope.
Female 3/5 > 12h,  < 24h Anorexia, Ataxia, hypoactivity, syncope, 5/5 > 1h,  < 2h Anorexia, Ataxia, hypoactivity, Lethargy, asthenia, convulsions, syncope.
4 Male 2/5 > 12h,  < 24h diarrhea with stools and urine deeply yellow, atypical locomotion (back limbs falling), syncope. 5/5 > 1h,  < 2h Anorexia, Ataxia, hypoactivity, Lethargy, asthenia, convulsions, syncope.
Female 5/5 > 12h,  < 24h Anorexia, Ataxia, hypoactivity, Lethargy, asthenia, convulsions 5/5 > 1h,  < 2h Anorexia, Ataxia, hypoactivity, Lethargy, asthenia, convulsions, syncope.

D/T = dead/treated mice; none = no toxic symptoms during the observation period; mortality latency = time to death (in hours) after the injection. Latency time elapsed between administration and death.