Fig. 2.

CyTOF profiling analyzes immune cells in chronically infected lung tissues. a Study design: Lungs were collected from 4T1-bearing mice with or without chronic PAO1 infection at the endpoint (21 days) and were analyzed by CyTOF. Unique immune subsets were identified by CyTOF analysis (n = 2 mice/group). b Two-dimensional t-SNE illustration of the merged CyTOF data from the lungs of both the CTRL group and CI group. c Frequency of immune lineages based on summation of Phenograph metaclusters. Composition of the CD45⁺ compartment showing average frequencies of major immune lineages in the lung tissue from the CTRL group and CI group (n = 2 mice/group). d viSNE analysis of lymphoid cells colored and labeled by Phenograph metacluster for the two groups. e Normalized expression of PD-1 in lung CD3⁺ cells shown by the viSNE plot for the CTRL and CI groups. f viSNE analysis of myeloid cells colored and labeled by Phenograph metacluster for the two groups