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. 2023 Aug 10;13:12279. doi: 10.1038/s41598-023-39196-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Wright–Giemsa staining of erythroid cells from wild-type and klf1-klf17 mutant embryos. (A, B) Wright–Giemsa staining at 52 hpf. Erythroid cells in the klf1-klf17 mutant (B) had larger nuclei and more basophilic cytoplasm than wild-type cells (A) at 52 hpf. Scale bar, 10 μm (A). Genotyping was performed by genomic PCR using locus-specific primers. (C) Scatter plots of the nucleus-to-cytoplasm (N:C) ratio in the wild-type (n = 6) and the klf1-klf17 mutant (n = 4) at 52 hpf. The N:C ratio in the klf1-klf17 mutant was larger than that in the wild-type. The central horizontal lines indicate the mean values. The data shown are the mean ± standard deviation (SD). Each wild-type and klf1-klf17 mutant sample contained 10 cells. P < 0.001 was considered significant.