Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Aug 11;11:43. doi: 10.1038/s41413-023-00277-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Identification of an intronic enhancer within the TNFSF11 gene locus in osteocytic cells. a H3K4me3, CTCF and H3K27ac ChIP-seq profiles in human osteocytic cells (GSE188004, GSE175159, GSE187956),²² chondrocytes (GSE188124),²² fibroblasts (GSE128642),⁵⁶ and lymphocytes (ENCFF021TWR) and RNA Pol II ChIA–PET results (ENCFF674MPM) in WTC11 cells within the TNFSF11 gene locus. The blue and red shaded areas indicate the RANKL promoter region and intronic enhancer region, respectively. Genetic variants associated with bone mineral density (P < 5 × 10^–6) identified in previous studies^25–28 that are located in the RANKL promoter-intronic enhancer loop are indicated in red letters. b Schematic depicting the enriched transcription factor-binding motifs and the predicted binding sites within the intronic enhancer region (upper). ChIP-seq profiles of C/EBPβ (ENCFF679XUJ), C/EBPα (ENCFF988QGQ), CREB1 (ENCFF894ALC) and GATA4 (ENCFF213XUO) (lower). c,d Protein‒protein interaction network (c) and functional enrichment analysis (d) of the predicted TFs using the STRING database⁵⁸ (https://string-db.org)