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. 2023 Jun 30;27(8):4785–4794. doi: 10.1007/s00784-023-05107-x

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Interleukin expressions of HGF after exposure to IL1β + TNFα treatment or HSC2 lysates. A For gene expressions, HGF treated with supernatant control showed significantly less IL1β, IL6, and IL8 expressions than the treatment with the positive control for inflammation (IL1β + TNFα). On the other hand, HGF treated with sonication and freeze/thawing lysates expressed IL1β, IL6, and IL8 to similar levels of the IL1β + TNFα treatment. B IL6 immunoassay confirmed the elevated presence of interleukin produced by all groups in this in vitro model for inflammation. In the graphs, different symbol shapes mean independent experiments. * means p < 0.05 and ** means p < 0.01