Fig. 3 (Figure 3 is missing, the file is in the attachment).

Culture supernatants were withdrawn and washed 24 h after LPS treatment and replenished with a fresh treatment medium to remove LPS and proinflammatory factors produced. At various time points after washout, the expression of A C3 mRNA and B protein was detected in neuron/glia cultures (N/G). C C3 mRNA in C57BL/6 J neuron/glia cultures was measured on day 3 after damage-associated molecular patterns (DAMPs) conditioned medium treatment. D Western blot analysis for C3 expression and quantified data in C57BL/6 J NG on day5 after DAMPs treatment. The reason for measuring mRNA and protein at different time points is to allow a sufficient period for the translation of mRNA to protein. All results are mean ± SEM from at least three independent experiments in duplicate. *p < 0.05, **p < 0.01, and *** p < 0.001. Panels A and B were analyzed with one-way ANOVA followed by Dunnett’s post hoc multiple comparisons test. Panels C and D were analyzed with unpaired t-test