Fig. 4.

LPS and proinflammatory factors were washed out and a fresh medium was added back to the cell cultures 24 h after LPS treatment. C3 protein was determined by A Western blot in C57BL/6 J and Mac1-deficient neuron/glia cultures. B Immunocytochemical staining and quantification of C3 expression in C57BL/6 J and Mac1 KO neuron/glia cultures treated with 15 ng/ml LPS (LPS was washed out at 24 h after treatment). Scale bar: 50 μm. All results are mean ± SEM from at least three independent experiments in duplicate. **p < 0.01. Data were analyzed with two-way ANOVA followed by Sidak’s post hoc multiple comparisons test