Fig. 5.

A C3 protein expression was detected by Western blot in C57BL/6 J and NOX2-deficient (NOX2 KO) neuron/glia cultures (N/G). Western blot was qualified by densitometry. B Immunostaining of C3 expression of C57BL/6 J neuron/glial cultures treated with LPS and LPS plus DPI (10⁻¹³ M), a NOX2 inhibitor. Scale bar: 50 μm. C C57BL/6 J and NOX-2 KO mice were treated with LPS (3.5 mg/kg i.p.). Brain C3 mRNA in C57BL/6 J and NOX2-deficient mice 24 h after LPS injection. D Brain C3 mRNA levels in LPS and LPS + DPI injected C57BL/6 J mice were measured 24 h after LPS injection. We pre-treated C57BL/6 J mice with saline or 10 ng/kg DPI for 4 days by subcutaneous injection (3 times per day) before the single LPS administration, then continuously post-treated at 6 and 12 h after LPS injection. All brain samples were collected 24 h after LPS to determine the brain C3 mRNA level. There are at least three mice in each group. All results are mean ± SEM from at least three independent experiments in duplicate. *P < 0.05, **P < 0.01. Panels A and C were analyzed with two-way ANOVA followed by Sidak’s post hoc multiple comparisons test. Panels B and D were analyzed with one-way ANOVA followed by Dunnett’s post hoc multiple comparisons test