Fig. 6.

C3 increases the production of superoxide-mediated oxidative stress and enhances neuron damage through the activation of the Mac1-NOX2 axis. A Immunocytochemical staining of 3-nitrotyrosine (3-NT), an oxidative stress marker, in C57BL/6 J, and C3 KO neuron/glia cultures at 7 days after LPS treatment. All results are mean ± SEM from at least three independent experiments in duplicate. *p < 0.05 and **p < 0.01. B Superoxide level was detected in endotoxin-free iC3b (500 ng/ml) treated C57BL/6 J mixed glial cultures. Panel A was analyzed with two-way ANOVA flowed by Sidak’s post hoc multiple comparisons test. Panel B was analyzed with an unpaired t-test