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. Author manuscript; available in PMC: 2023 Oct 23.
Published in final edited form as: Cell Rep. 2023 Feb 13;42(2):112120. doi: 10.1016/j.celrep.2023.112120

Figure 5. The potential role of polyamines in H. parainfluenzae S1p strain pathogenicity.

Figure 5.

(A) Polyamine metabolism pathway. Cecal content polyamine concentrations in mice inoculated with S1p strain (n = 5) or PBS (n = 4) were measured using UPLC assay.

(B–D) Concentrations of polyamines were determined based on standard curves and normalized to protein concentrations of (B) putrescine (PUT), (C) spermidine (SPD), and (D) spermine (SPM).

(F and G) Probe-base qPCR assay was performed on proximal colon samples to measure the transcription activity of ornithine decarboxylase 1 (Odc1), (F) deoxyhypusine synthase (Dhps), and (G) SPM oxidase (Smox) relative to TATA box binding protein (Tbp) housekeeping gene.

Statistical testing was performed using a two-sample t test. Data are represented as mean ± SEM.