Figure 7.

MCC950 treatment improves autophagy signaling. Immunostaining for autophagy marker LC3 (A–D), for the ER stress response protein GRP94 (E–H) and the mTOR signaling readout pS6RP (I–L) comparing young to middle-aged saline- and MCC950-treated mice (I–L). Panels I–K were counterstained with Lotus Tetragonolobus Lectin (LTL, blue) to visualize proximal tubules. Abbreviation: BC: Bowman’s Capsule. Images were quantified and depicted as % tuft area Scale bars in the images correspond to 25 μm.