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. 2023 Aug 10;12(8):12341. doi: 10.1002/jev2.12341

FIGURE 7.

FIGURE 7

Lymphatic CXCL8 secretion upon EV uptake leads to NETs formation. (a)Schematic illustration of the in vitro NETosis assay induced by CM of treated LECs. (b) Representative images of neutrophils treated with CM from treated LECs. Scale bars represent 20 μm. (c) Quantification of LEC CM induced NETosis (% of H3Cit positive neutrophils). (d) Representative images of indicating the different proneness to form NETs for neutrophils from health control and GEA patients after A549 EV treatment. Scale bars represent 100μm. (d) Quantification of propensity of neutrophil to form NETs induced by EVs (Relative fluorescence intensity of SYTOX Green treated to untreated neutrophil) from health control and GEA patients). Data shown as mean ± SEM.**, P < 0.01; ****, P < 0.001 by Kruskal–Wallis test or Mann–Whitney t test.