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. 2023 Jul 7;299(8):105021. doi: 10.1016/j.jbc.2023.105021

Figure 2.

Figure 2

Patient-derived organoid cultures retain estrogen receptor (ER) expression during long-term culture and resemble original patient tumors histology.A, representative brightfield images and ER immunohistochemistry of established HR+ PDO lines. B, brightfield images of H&E stained matching patient tumor and PDOs. C, table showing mutations found in the original patient tumors and at matching PDOs isolated from these tumors. Patient #8 and #10 had prior mutational analysis performed in their tumors. PDOs were exome sequenced to identify matching mutations. D, representative confocal images of patient #8 PDOs stained for ER (green) and nuclei (DAPI: blue) at passage 5 and passage 15. The scale bars represent 200 μm (A), 100 μm (B), and 40 μm (D). DAPI, 4′,6-diamidino-2-phenylindole; ER, estrogen receptor; HR+, hormone receptor–positive; PDOs, patient-derived organoids.