Fig. 2.

Optimisation of IgG enzymatic immunoassays (EIAs) using HEV-A4 p239 and HEV-C1 p241.

Checkerboard plots of HEV-A4 p239 (A) and HEV-C1 p241 (B) EIAs for optimising antigen coating concentrations and sample dilutions. Five HEV-C1 and five HEV-A RT-PCR-positive plasma samples were chosen for initial EIA optimisation. Two seronegative donor samples served as negative controls. Error bars represent mean and standard deviation of optical densities at denoted antigen coating concentrations and sample dilution. Samples were tested in triplicate. HEV-A, Paslahepevirus balayani; HEV-C1, Rocahepevirus ratti.