Table 1.

Antimicrobial screening of compound 2 and 3 against E. coli and S. aureus

Entry	Compound. #	Substituents		Inhibitory screening				Dose response screening
		NMM	DABCO	E. coli @ 150 μg/mL	E. coli @ 300 μg/mL	S. aureus @ 100 μg/mL	S. aureus @ 200 μg/mL	S. aureus IC50 (μg/mL)
1	2a	4-BrPh	–	–	–	+++	+++	20
2.	2c	4-t-BuPh	–	–	–	+++	+++	<200
3.	2g	4-ClPh	–	–	–	+++	+++	80
4.	2h	3-BrPh	–	–	–	+++	+++	90
5.	2i	3-ClPh	–	–	–	+++	+++	<200
6.	2j	3-FPh	–	–	–	+++	+++	<200
7.	2k	3-CF3Ph	–	–	–	+++	+++	<200
8.	2o	3-thienyl	–	–	–	+++	+++	40
9.	3a	–	4-anisolyl	–	–	+++	+++	<200
10.	3b	–	4-t-BuPh	–	–	+++	+++	<200
11.	3c	–	4-FPh	–	–	+++	+++	<200
12.	3d	–	3-anisolyl	–	–	+++	+++	5
13.	3e	–	3-ClPh	–	–	+++	+++	80
14.	3f	–	3-CF3Ph	–	–	+++	+++	10
15.	3g	–	4-ClPh	–	–	+++	+++	20
16.	3i	–	3-FPh	–	–	+++	+++	<200
17	CPC	–	–	–	–	+++	+++	∼20041

--- represents no inhibition effect of compounds on growth as O.D._600nm above 1 was observed.

+++ represents the inhibition effect of compounds on the growth of S. aureus cells.

Mention about the statistical analysis – IC₅₀ for each compound against S. aureus was obtained by calculating the concentrations corresponding to percentage inhibition mean values.