Table 2. Allele frequencies in El Pachón cave.

H-c5	Lab	2016	2019	2022 (1)	2022 (2)	I-b1	Lab	2016	2019	2022 (1)	2022 (2)	V-f3	Lab	2016	2019	2022 (1)	2022 (2)
For each of the 18 loci analyzed, allele frequencies are shown for each session of sampling in the lab (2018+2021) and in the cave (2016, 2019 and 2022). Yellow highlighting indicates the frequency of dominant alleles over the years. Lab stock was obtained with fish sampled before 2004. He: Expected heterozygosity.
154	1,00	0.97	0.99	0.98	0.98	305		0.10	0.06	0.08	0.10	213			0.01	0.02
172		0.03	0.01	0.02	0.02	311	0.09	0.03	0.04	0.02	0.02	215	1,00	1,00	0.99	0.98	1,00
						313	0.91	0.87	0.90	0.90	0.88
He	0.00	0.05	0.03	0.03	0.03	He	0.17	0.23	0.18	0.19	0.22	He	0.00	0.00	0.03	0.03	0.00
A8-g5	Lab	2016	2019	2022 (1)	2022 (2)	X-a3	Lab	2016	2019	2022 (1)	2022 (2)	W-f6	Lab	2016	2019	2022 (1)	2022 (2)
160		0.45	0.31	0.33	0.29	202	0.81	0.74	0.81	0.67	0.71	289	1,00	1,00	0.97	0.98	1,00
168	1,00	0.55	0.69	0.67	0.67	206		0.03	0.03	0.02		295			0.03	0.02
176					0.04	208	0.19	0.23	0.16	0.31	0.29
He	0.00	0.49	0.43	0.44	0.46	He	0.30	0.40	0.31	0.45	0.41	He	0.00	0.00	0.06	0.03	0.00
A13-e5	Lab	2016	2019	2022 (1)	2022 (2)	L-b9	Lab	2016	2019	2022 (1)	2022 (2)	A2-a7	Lab	2016	2019	2022 (1)	2022 (2)
242			0.03	0.02	0.03	213	0.34		0.09	0.05	0.05	106					0.02
246		0.03		0.02		225	0.66	1,00	0.91	0.95	0.95	108	0.56	0.24	0.13	0.12	0.19
252					0.03							112		0.37	0.32	0.33	0.40
254	1,00	0.97	0.97	0.96	0.91							114	0.28	0.34	0.44	0.45	0.36
256					0.03							124	0.16	0.05	0.11	0.10	0.03
He	0.00	0.05	0.06	0.07	0.16	He	0.45	0.00	0.16	0.10	0.10	He	0.58	0.69	0.68	0.67	0.67
V-c10	Lab	2016	2019	2022 (1)	2022 (2)	A13-f8	Lab	2016	2019	2022 (1)	2022 (2)	A6-f1	Lab	2016	2019	2022 (1)	2022 (2)
228			0.04	0.02	0.03	121		0.05		0.02		265	0.16	0.41	0.14	0.20	0.31
246		0.03	0.04	0.05		125	1,00	0.95	0.88	0.93	0.98	267			0.05	0.02
248	1,00	0.97	0.92	0.93	0.97	127			0.06	0.02		271	0.84	0.59	0.81	0.78	0.69
						135			0.06	0.03	0.02
He	0.00	0.05	0.16	0.13	0.07	He	0.00	0.10	0.21	0.13	0.03	He	0.26	0.49	0.31	0.35	0.43
A6-h6	lab	2016	2019	2022 (1)	2022 (2)	A14-d8	lab	2016	2019	2022 (1)	2022 (2)	A4-g11	lab	2016	2019	2022 (1)	2022 (2)
103	1,00	0.97	0.99	0.98	0.97	129	1,00	1,00	1,00	1,00	1,00	165	1,00	1,00	0.94	0.98	0.98
115		0.03	0.01	0.02	0.03							171			0.06	0.02	0.02
He	0.00	0.05	0.03	0.03	0.07	He	0.00	0.00	0.00	0.00	0.00	He	0.00	0.00	0.11	0.03	0.03
W-d11	lab	2016	2019	2022 (1)	2022 (2)	W-c12	lab	2016	2019	2022 (1)	2022 (2)	A5-f9	lab	2016	2019	2022 (1)	2022 (2)
162	0.03	0.16	0.19	0.14	0.22	173		0.03	0.01	0.03	0.07	198	1,00	0.97	0.96	1,00	0.97
166		0.18	0.33	0.26	0.21	177			0.01		0.02	200		0.03	0.04		0.03
172		0.08				185	1,00	0.97	0.98	0.97	0.91
176	0.19	0.24	0.09	0.22	0.09
186	0.75	0.32	0.37	0.38	0.45
188	0.03	0.02	0.01
200			0.01		0.03
He	0.40	0.78	0.71	0.72	0.70	He	0.00	0.05	0.06	0.07	0.16	He	0.00	0.05	0.08	0.00	0.07