FIG. 1.
(A) Schematic representation of the DNA sequence arrangements of the HSV-1 genome of new recombinant viruses used in this study. Top line, linear representation of the HSV-1 genome. The open rectangles represent the terminal repeats flanking the unique long (UL) and unique short (US) sequences. The location of the α22/US1.5 gene is shown. Line 1, representation of the BamHI N fragment which contains the α22/US1.5 gene. The transcript is represented by the arrow, and the coding domain of the α22/US1.5 genes is represented by the open rectangle. Line 2, recombinant R7820, in which the C-terminal 22 amino acids encoded by the α22 open reading frame have been deleted. Line 3, recombinant R7810, in which the C-terminal 40 amino acids encoded by the α22 open reading frame have been deleted. B, BamHI. (B) Polypeptide sequence of the carboxyl terminus of ICP22. Line 1, the 43 amino acids of the carboxyl-terminal domain of ICP22. Line 2, sequence of the 21 amino acids remaining within the carboxyl-terminal domain of R7820. Line 3, the 3 amino acids remaining within the carboxyl-terminal domain of R7810.