FIG. 3.

Photographic image of cell proteins bound to GST fusion protein, electrophoretically separated in a denaturing gel, and reacted with antiserum to ICP22. Fusion proteins were grown in BL21 cells and purified as recommended by the manufacturer (Pharmacia). GST and GST-p60 were mixed with HSV-1(F)-infected HeLa extract, and beads were collected and washed as described in Materials and Methods. Proteins were separated in 7% denaturing polyacrylamide gel, transferred to nitrocellulose, blocked, reacted with R77 and then with a goat anti-rabbit antibody conjugated to horseradish peroxidase, and processed as described by the manufacturer (Amersham). Lanes: 1, cell extract from HSV-1(F)-infected HeLa cells; 2 and 3, infected HeLa cell extract bound to GST and GST-p60, respectively.