Figure 4.

The phenotype of Dmp1-driven CRISPRi progresses with age

(A–C) BMD of female Dmp1^dCas9::KRAB;sgRNA^Tnfsf11 mice (Ot-CRi^Tnfsf11) and their littermate controls (wild type, Dmp1^dCas9::KRAB, and sgRNA^Tnfsf11 mice) were measured at 4 and 12 months of age. For the 4-month cohort n = 8–17 mice/group, and for the 12-month cohort n = 2–12 mice/group were used. Data presented as mean ± SD. For both ages #,p < 0.05 comparing Ot-CRi^Tnfsf11 with each control group using one-way ANOVA with Tukey adjustment. Individual Tukey p values for each comparison are provided in the graphs.∗, p < 0.05 for comparison of the change with age (between 12 and 4 months) for the Ot-CRi^Tnfsf11 group and the change with age within the control groups using t-test (details explained in the Methods Statistics section).