FIG. 5.

Model RNA-derived gene expression exceeds HV gene expression. SDI-CL(NP) was passaged with SAD Ambi as described above for SAD Ambi-CAT. For the third passage, BSR cells were infected with the HV-DI stock (Ambi; +) at an HV MOI of 1. Cells were also infected with SAD Ambi alone (Ambi; −) or with SAD Ambi-CAT (Ambi-CAT) at an MOI of 1. Cell RNA was analyzed by Northern blotting 2 days postinfection by CAT- and RV N gene-specific DNA probes. In SAD Ambi–SDI-CL(NP)-coinfected cells, the level of monocistronic CAT mRNA was 11-fold higher than in cells infected with SAD Ambi-CAT. The level of N mRNA was fourfold lower in SAD Ambi–SDI-CL(NP)-coinfected cells than in SAD Ambi- or SAD Ambi-CAT-infected cells. luc, luciferase.