B6, B6.gld, and B6.lpr mice were immunized with 106 B.GV tumor cells. At 12 days later, 107 responder spleen cells were placed in MLTC with 2 × 105 E♂G2 tumor stimulator cells and/or 2 × 106 viable AKR.H-2b splenocytes, as indicated. At 6 days later, the cells were assayed for the ability to lyse 51Cr-labeled E♂G2 tumor target cells. The value for spontaneous release by target cells was 12.1%. E:T, effector-to-target-cell ratio.