Figure 5.

proNGF induced EGFR internalization is dependent of sortilin. (A) Colocalization of EGFR/Rab5 assessed by immunofluorescence. The confocal laser scanning microscope settings were maintained between all conditions. EGFR/Rab5 colocalization was assessed on Cal 27 wt and Cal 27 cells with inhibition of SORT1 expression by siRNA. Green fluorochrome corresponded to EGFR (AlexaFluor 488), red to Rab5 (AlexaFluor 546) and white to the colocalization channel. (B) The scatter plots showed the results of the calculation of the Manders weighted overlap coefficient for the red channel in each condition (10 images coefficients are compared using a non-parametric Mann-Whitney test (*p<0.05, **p<0.01, ***p<0.001, ns, not significant)).