. 2023 Jul 22;12(7):1221. doi: 10.3390/antibiotics12071221

Table 1.

Virulence genes primers used in this study, target gene, and sequence.

Gene Target	5′—Oligo Seq—3′	Product Size (bp)	Annealing Temp. (°C)	Ref.
fnbA-F	CACAACCAGCAAATATAG	1279	48	[17]
fnbA-R	CTGTGTGGTAATCAATGTC	1279	48	[17]
fnbB-F	CAGAAGTACCAAGCGAGCCGGAAA	258	65	[16]
fnbB-R	CGAACAACATGCCGTTGTTTGTTGA	258	65	[16]
clfA-F	ATTGGCGTGGCTTCAGTGCTTG	357	48	[16]
clfA-R	GCTTGATTGAGTTGTTGCCGGTGT	357	48	[16]
sdrC-F	CGCATGGCAGTGAATACTGTTGCAGC	731	50	[17]
sdrC-R	GAAGTATCAGGGGTGAAACTATCCACAAATTG	731	50	[17]
sdrD-F	CCACTGGAAATAAAGTTGAAGTTTCAACTGCC	467	61	[17]
sdrD-R	CCTGATTTAACTTTGTCATCAACTGTAATTTGTG	467	61	[17]
spa-F	TCGAAATAGCGTGATTTTGC	polymorphism	61	[17]
spa-R	GCACTGAGCAACAAAAGATG	polymorphism	61	[17]
pvl-F	TGCCAGACAATGAATTACCCCCATT	894	60	[17]
pvl-R	TCTGCCATATGGTCCCCAACCA	894	60	[17]
sea-F	TTGCAGGGAACAGCTTTAGGCAATC	252	60	[17]
sea-R	TGGTGTACCACCCGCACATTGA	252	60	[17]
seb-F	GACATGATGCCTGCACCAGGAGA	355	64	[17]
seb-R	AACAAATCGTTAAAAACGGCGACACAG	355	64	[17]
sec-F	CCCTACGCCAGATGAGTTGCACA	602	62	[17]
sec-R	CGCCTGGTGCAGGCATCATATC	602	62	[17]
sed-F	GAAAGTGAGCAAGTTGGATAGATTGCGGCTAG	362	65	[17]
sed-R	CCGCGCTGTATTTTTCCTCCGAGAG	362	65	[17]
agr I-F	ATCGCAGCTTATAGTACTTGT	814	55	[16]
agr I-R	CTTGATTACGTTTATATTTCATC	814	55	[16]
efb-F	CGGTCCAAGAGAAAAGAAACCAGTGAG	276	63	[17]
efb-R	TGTGCTTTTCTGTGTGCACTGACAGTATG	276	63	[17]
icaA-F	TCAGACACTTGCTGGCGCAGTC	936	55	[17]
icaA -R	TCACGATTCTCTCCCTCTCTGCCATT	936	55	[17]
tst-F	AGCCCTGCTTTTACAAAAGGGGAAAA	306	64	[17]
tst-R	CCAATAACCACCCGTTTTATCGCTTG	306	64	[17]
hlg-F	TTGGCTGGGGAGTTGAAGCACA	306	64	[17]
hlg-R	CGCCTGCCCAGTAGAAGCCATT	306	64	[17]
hla-F	CGAAAGGTACCATTGCTGGT	744	53	[18]
hla-R	CCAATCGATTTTATATCTTTC	744	53	[18]
coa -F	ATAGAGATGCTGGTACAGG	polymorphism	58	[17]
coa-R	GCTTCCGATTGTTCGATGC	polymorphism	58	[17]
nuc-F	GCGATTGATGGTGATACGGTT	279	60	[17]
nuc-R	AGCCAAGCCTTGACGAACTAAAGC	279	60	[17]