Table 2.
Gene Target | 5′—Oligo Seq—3′ | Product Size (bp) | Annealing Temp. (°C) | Ref. |
---|---|---|---|---|
blaZ-F | ACTTCAACACCTGCTGCTTTC | 173 | 54 | [14] |
blaZ-R | TGACCACTTTTATCAGCAACC | |||
mecA-F | CCTAGTAAAGCTCCGGAA | 314 | 55 | [14] |
mecA-R | CTAGTCCATTCGGTCCA | |||
tetK-F | GTAGCGACAATAGGTAATAGT | 360 | 54 | [14] |
tetK-R | GTAGTGACAATAAAC CTCCTA | |||
tetM-F | AGTGGAGCGATTACAGAA | 158 | 54 | [14] |
tetM-R | CATATGTCCTGG CGTGTCTA | |||
ermA-F | AAGCGGTAAACCCCTCTG A | 190 | 58 | [14] |
ermA-R | TTC GCAAATCCCTTCTCAAC | |||
ermB-F | CTATCTGATTGTTGAAGAAGGATT | 142 | 54 | [14] |
ermB-R | GTTTACTCTTGGTTTAGGATGAAA | |||
ermC-F | AAT CGTCAA TTCCTG CAT GT | 299 | 54 | [14] |
ermC-R | TAATCG TGGAATACGGGTTTG | |||
16S-F | AGA GTT TGA TCC TGG CTC AG | 1500 | 52 | [19] |
16S-R | AAG GAG GTG ATC CAG CCG CA |