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. 2023 Jul 22;12(7):1221. doi: 10.3390/antibiotics12071221

Table 2.

Primers sequence specific to different antimicrobials resistant determinants in S. aureus.

Gene Target 5′—Oligo Seq—3′ Product Size (bp) Annealing Temp. (°C) Ref.
blaZ-F ACTTCAACACCTGCTGCTTTC 173 54 [14]
blaZ-R TGACCACTTTTATCAGCAACC
mecA-F CCTAGTAAAGCTCCGGAA 314 55 [14]
mecA-R CTAGTCCATTCGGTCCA
tetK-F GTAGCGACAATAGGTAATAGT 360 54 [14]
tetK-R GTAGTGACAATAAAC CTCCTA
tetM-F AGTGGAGCGATTACAGAA 158 54 [14]
tetM-R CATATGTCCTGG CGTGTCTA
ermA-F AAGCGGTAAACCCCTCTG A 190 58 [14]
ermA-R TTC GCAAATCCCTTCTCAAC
ermB-F CTATCTGATTGTTGAAGAAGGATT 142 54 [14]
ermB-R GTTTACTCTTGGTTTAGGATGAAA
ermC-F AAT CGTCAA TTCCTG CAT GT 299 54 [14]
ermC-R TAATCG TGGAATACGGGTTTG
16S-F AGA GTT TGA TCC TGG CTC AG 1500 52 [19]
16S-R AAG GAG GTG ATC CAG CCG CA