Skip to main content
. 2023 Jul 25;79(Pt 8):200–207. doi: 10.1107/S2053230X23005721

Table 2. Crystallization.

Method Vapor diffusion
Plate type Sitting drop
Temperature (K) 293
Protein concentration 4
Buffer composition of protein solution 10 mM Tris–HCl pH 7.5, 200 mM NaCl, 2 mM MgCl2
Composition of reservoir solution 20%(w/v) PEG 4000, 100 mM MES pH 6.5, 5 mM MgCl2
Volume and ratio of drop 2 µl, 1:1 ratio
Volume of reservoir (µl) 500